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APC anti-human CD16 Antibody
产品名称：
APC anti-human CD16 Antibody
产品类别：
抗体
产品编号：
302011
产品应用：
302011
[价格]
规格 价格 库存
25tests ￥ 1584 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD16 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-S?rensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Guo Y, et al. 2021. Exp Ther Med. 353:21. PubMed
  2. Rizzo MD, et al. 2019. J Pharmacol Exp Ther. 371:191. PubMed
  3. Cruz MA, et al. 2022. Nat Nanotechnol. 17:1004. PubMed
  4. Gopinath A, et al. 2020. J Immunol Methods. 476:112686. PubMed
  5. Meya D, et al. 2015. J Infect Dis. 211:1597. PubMed
  6. Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed
  7. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  8. Pazina T, et al. 2019. Cancer Immunol Res. 7:1633. PubMed
  9. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  10. Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed
  11. Wang H, et al. 2020. Onco Targets Ther. 13:3903. PubMed
  12. Hünniger K, et al. 2015. J Immunol. 194:1199. PubMed
  13. Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed
  14. Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed
  15. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  16. Paris O, et al. 2022. Front Immunol. 13:975910. PubMed
  17. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  18. Guo L, et al. 2019. Breast Cancer Res Treat. 175:459. PubMed
  19. Kho D, et al. 2017. PLoS One. 10.1371/journal.pone.0180267. PubMed
  20. Xia Y, et al. 2019. Gastroenterol Res Pract. 2019:5436961. PubMed
  21. González-Tajuelo R, et al. 2020. Front Immunol. 11:588212. PubMed
  22. EC O, et al. 2015. Sci Rep. 5: 11888. PubMed
  23. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  24. Ge S, et al. 2012. Nephrol Dial Transplant. 209:35:00. PubMed
  25. Saha R, et al. 2021. Sci Rep. 11:18849. PubMed
  26. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  27. Chen J, et al. 2017. J Immunol. 10.4049/jimmunol.1700159. PubMed
  28. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  29. Rizzo MD, et al. 2018. AIDS. 32:419. PubMed
  30. Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed
  31. Motwani MP, et al. 2018. JCI Insight. 3:e94463. PubMed
  32. Wang H, et al. 2011. Immunol Lett. 136:21. PubMed
  33. Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
  34. Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed
  35. Hiatt J, et al. 2021. Cell Reports. 35(6):109105. PubMed
  36. Yabe IM, et al. 2018. Mol Ther Methods Clin Dev. 11:143. PubMed
  37. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  38. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  39. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  40. Tamminga M, et al. 2020. Transl Lung Cancer Res. 1.134027778. PubMed
RRID
AB_314211 (BioLegend Cat. No. 302011) AB_314212 (BioLegend Cat. No. 302012)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

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