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Alexa Fluor® 700 anti-human CD16 Antibody
产品名称：
Alexa Fluor® 700 anti-human CD16 Antibody
产品类别：
抗体
产品编号：
302025
产品应用：
302025
[价格]
规格 价格 库存
25µg ￥ 1488 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-S?rensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Carre C, et al. 2021. iScience. 24:102970. PubMed
  2. Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
  3. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  4. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  5. Koutsakos M, et al. 2021. Cell Reports Medicine. 2(3):100208. PubMed
  6. Veenhuis RT, et al. 2021. JCI Insight. 6:. PubMed
  7. de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
  8. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  9. Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
  10. Bj?rklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  11. Chalan P, et al. 2015. PLoS One. 10: 0132436. PubMed
  12. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  13. Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed
  14. Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed
  15. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  16. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  17. Lusty E, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.025. PubMed
  18. Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed
  19. Vaccari M, et al. 2013. J Virol. 87:3526. PubMed
  20. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  21. Georg P, et al. 2022. Cell. 185:493. PubMed
  22. NULL, et al. 2022. Cell. 185:916. PubMed
  23. Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed
  24. Wimmers F, et al. 2021. Cell. 184:3915. PubMed
  25. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  26. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  27. Pate K, et al. 2015. J Infect Dis. 212: 1387 - 1396. PubMed
  28. Connors J, et al. 2022. Geroscience. :. PubMed
  29. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  30. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  31. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  32. Chowdhury A, et al. 2015. J Virol. 89: 8677-8686. PubMed
RRID
AB_493748 (BioLegend Cat. No. 302025) AB_2278418 (BioLegend Cat. No. 302026)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

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