PE/Cyanine7 anti-human CD210 (IL-10 R) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2112	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

shIL-10R

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.⁸ Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro blocking^1-3 of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for highly sensitive assays?(Cat. No. 308817 and 308818).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Liu Y, et al. 1997. J. Immunol. 158:604. (Immunogen, IP, Block)
Levings MK, et al. 2005. Blood 105:1162. (Block)
Goodier MR, et al. 2000. J. Immunol. 165:139. (Block)
Huang YH, et al. 2009. J. Leukoc. Biol. 86:273. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Liu BS,et al. 2011. J Leukoc Biol. 89:981. PubMed
Joffe M, et al. 2012. Int Immunol. 24:447. PubMed
MacDonald KP, et al. 1999. J. Immunol. 163:5599. (epitope)

Product Citations

Aung Naing et al. 2018. Cancer cell. 34(5):775-791 . PubMed
Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed

RRID

AB_2565630 (BioLegend Cat. No. 308813) AB_2565631 (BioLegend Cat. No. 308814)

Antigen Details

Structure: Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD
Distribution: T cells and B cells, NK cells, monocytes, macrophages
Function: Activates STAT1, STAT3, Jak1, Tyk
Ligand/Receptor: IL-10
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Kotenko S. 2002. Cytokine Growth Factor Rev. 13:223.
2. Trinchieri G. 2003. Nat. Rev. Immunol. 3:133.
Gene ID: 3587 View all products for this Gene ID
UniProt: View information about CD210 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们