Brilliant Violet 421™ anti-human CD210 (IL-1-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2244	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

shIL-10R

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.⁸ Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro blocking^1-3 of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for highly sensitive assays?(Cat. No. 308817 and 308818).

Application References (PubMed link indicates BioLegend citation)

Liu Y, et al. 1997. J. Immunol. 158:604. (Immunogen, IP, Block)
Levings MK, et al. 2005. Blood 105:1162. (Block)
Goodier MR, et al. 2000. J. Immunol. 165:139. (Block)
Huang YH, et al. 2009. J. Leukoc. Biol. 86:273. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Liu BS,et al. 2011. J Leukoc Biol. 89:981. PubMed
Joffe M, et al. 2012. Int Immunol. 24:447. PubMed
MacDonald KP, et al. 1999. J. Immunol. 163:5599. (epitope)

RRID

AB_2728246 (BioLegend Cat. No. 308815) AB_2728247 (BioLegend Cat. No. 308816)

Antigen Details

Structure: Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD
Distribution: T cells and B cells, NK cells, monocytes, macrophages
Function: Activates STAT1, STAT3, Jak1, Tyk
Ligand/Receptor: IL-10
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Kotenko S. 2002. Cytokine Growth Factor Rev. 13:223.
2. Trinchieri G. 2003. Nat. Rev. Immunol. 3:133.
Gene ID: 3587 View all products for this Gene ID
UniProt: View information about CD210 on UniProt.org

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