APC anti-human CD230 (Prion) Antibody
- 产品名称：
- APC anti-human CD230 (Prion) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 800315
- 产品应用：
- 800315
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1788 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoblotting, immunohistochemistry (IHC) on unfixed tissues, immunoprecipitation (IP) and ELISA.
3F4 is reactive to amino acid residues 109-112 of prion protein (PrP) from humans, hamsters, and felines. It recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).
- Application References
(PubMed link indicates BioLegend citation) -
- Kondoh G,?et al. 2005.?Nat. Med.?11(2):160-6.?(IHC-F)
- Gilch S,?et al. 2004.?Traffic. 5(4):300-13.?(WB, IHC-F, ELISA)?PubMed
- Nishina K,?et al.?2004.?Biochemistry?43:2613-21.?(IP)
- Shiga Y,?et al. 2004.?Neurology?63(3):443-9.?(WB, IHC-F, IP, ELISA)
- Zou WQ,?et al. 2004.?Proc. Natl. Acad. Sci. USA?101(5):1380-5.
- Kitamoto T,?et al. 1987.?Lab Invest.?57(2):230-6.
- Kascsak RJ,?et al. 1987.?J. Virol.?61(12):3688-93.
- RRID
- AB_2616653 (BioLegend Cat. No. 800315) AB_2616654 (BioLegend Cat. No. 800316)
- Biology Area
- Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Prion (CD230)
- Antigen References
-
1. Asante EA, et al. 2015. Nature 522:478.
2. Mead S, et al. 2013. N. Engl. J. Med. 369:1904.
3. Chakrabarti O, Hegde RS. 2009. Cell 137:1136.
4. Wadsworth JD, et al. 2004. Science 306:1793.
- Gene ID
- 5621 View all products for this Gene ID
- UniProt
- View information about CD230 on UniProt.org
