Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2
- 产品类别：
- 抗体
- 产品编号：
- 163903
- 产品应用：
- 163903
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Pre-B cell transfected with mCXCR2
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 3F10-B3 blocks the chemotaxis towards CXCR2 ligands.
Clone 3F10-B3 anti-mouse CXCR2 blocks the binding of CXCL2, inhibiting the migration of CXCR2+ cells towards CXCL2 in a dose dependent manner, with an ED50 between 1-4 ?g/mL.
- RRID
- AB_2927843 (BioLegend Cat. No. 163903) AB_2927843 (BioLegend Cat. No. 163904)
- Structure
- G-protein coupled receptor, 7 transmembrane regions
- Distribution
-
Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK)
- Function
- Chemotaxis, neutrophil activation, and angiogenesis
- Ligand/Receptor
- CXCL1, CXCL2, CXCL3, and CXCL5.
- Cell Type
- Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
- Spaan AN, et al. 2014. Nat?Commun. 5:5438.
- Natsuaki Y, et al. 2014. Nat?Immunol. 15:1064-9.
- Hsieh CY, et al. 2014. J?Immunol. 193:3693-703.
- Gene ID
- 12765 View all products for this Gene ID
- UniProt
- View information about CD182 on UniProt.org
