PE anti-HMGB1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1692	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human HMGB1 with GST tag. A universal T cell epitope from a Mycobacterium tuberculosis antigen was introduced into the C-terminus of HMGB1 to increase the immunogenicity.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Clone 3E8 has been reported to protect mice from LPS induced sepsis¹. Additional reported applications (for relevant formats) include: neutralization¹. The LEAF? or Ultra-LEAF? purified antibody is recommended for functional assays (contact our custom solutions team).?

Application References (PubMed link indicates BioLegend citation)

Zhou H, et al. 2009. PLos One 4:e6087. (Neut)

Product Citations

Liu X, et al. 2021. Nat Commun. 12:1832. PubMed
Florêncio KGD, et al. 2022. Front Immunol. 13:941757. PubMed
Coleman LG, et al. 2018. PLoS One. 13:e0195335. PubMed

RRID

AB_2562220 (BioLegend Cat. No. 651403) AB_2562221 (BioLegend Cat. No. 651404)

Antigen Details

Structure: 215 amino acids with predicted molecular weight of 25 kD.
Distribution: Nucleus
Function: DNA binding protein that associates with chromatin and has the ability to bend DNA. Binds single-stranded DNA preferentially. Involved in V(D)J recombination by acting as a cofactor of the RAG complex.
Interaction: Component of the RAG complex composed of core components RAG1 and RAG2.
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Thomas JO and Travers AA. 2001. Trends Biochem. Sci. 26:167.
2. Müller S, et al. 2004. J. Intern. Med. 255:332.
3. Campana L, et al. 2008. Curr. Opin. Immunol. 20:518.
4. Klune JR, et al. 2008. Mol. Med. 14:476.
5. Dumitriu IE, et al. 2005. Trends Immunol. 26:381.
6. Bonaldi T, et al. 2003. EMBO J. 22:5551.
Gene ID: 3146 View all products for this Gene ID
UniProt: View information about HMGB1 on UniProt.org

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