Purified anti-complement C3b/iC3b Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2748	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Non-Human Primate

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Purified native human C3b protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
Direct ELISA - Verified
Block, ELISA Detection, ICC, RIA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 2.0 - 4.0 ?g per ml. For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 ?g per ml.?It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Blocking³, ELISA Detection¹, immunocytochemistry^1,2, and RIA^2,5.

This antibody recognizes C3, C3b and iC3b, and does not cross-react with C3d.

Application References (PubMed link indicates BioLegend citation)

Pokrass M, et al. 2013. Mol. Immunol. 56:549. (ELISA, ICC)
Kennedy AD, et al. 2003. Blood. 101:1071. (FC, ICC, RIA)
DiLillo DJ, et al. 2006. Mol. Immunol. 43:1010-9.
Lindorfer MA, et al. 2010 Blood 115:2283. (Block)
Sokoloff MH, et al. 2000. Cancer Immunol. Immunother. 49:551. (RIA, FC)

RRID

AB_2571918 (BioLegend Cat. No. 846102)

Antigen Details

Structure: C3b is a glycosylated, 176 kD protein that is formed by the cleavage of C3. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins.
Distribution: Extracellular fluid, plasma membrane, and serum.
Function: C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b is less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
Interaction: C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors, CR1 (CD35), for C3b. iC3b can also function as opsonin, but it binds through CR2 and CR3.
Ligand/Receptor: C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b can interact with CR2 (CD21), and CR3 (CD11b/CD18).
Biology Area: Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Lambris JD. 1988. Immunol. Today 9:387-93.
Gene ID: 718 View all products for this Gene ID
UniProt: View information about C3b/iC3b on UniProt.org

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