PE/Cyanine7 anti-mouse CD169 (Siglec-1) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2112	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Native Sialoadhesin from spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections¹, and spatial biology (IBEX)^4,5.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
Chtanova T, et al. 2008. Immunity 29:487.
Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
Smith LK, et al. 2021. Elife. 10:. PubMed
Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
Lee A, et al. 2022. Nat Commun. 13:549. PubMed
Song X, et al. 2022. Transl Oncol. 15:101306. PubMed

RRID

AB_2563910 (BioLegend Cat. No. 142411) AB_2563911 (BioLegend Cat. No. 142412)

Antigen Details

Structure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function: Adhesion.
Ligand/Receptor: PSGL-1, CD43, CD206 and CD227.
Cell Type: Macrophages
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
Gene ID: 20612 View all products for this Gene ID
UniProt: View information about CD169 on UniProt.org

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