PE anti-mouse CD169 (Siglec-1) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1836	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Native Sialoadhesin from spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections¹, and spatial biology (IBEX)^4,5.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
Chtanova T, et al. 2008. Immunity 29:487.
Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Anania JC, et al. 2021. Front Immunol. 12:655753. PubMed
Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
Juul-Madsen K, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
Kato Y, et al. 2020. Immunity. 53(3):548-563. PubMed
Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
Beura LK, et al. 2018. Immunity. 48:327. PubMed
Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
Feuerstein R, et al. 2015. J Immunol. 194:2735. PubMed
Song X, et al. 2022. Transl Oncol. 15:101306. PubMed

RRID

AB_10915470 (BioLegend Cat. No. 142403) AB_10915697 (BioLegend Cat. No. 142404)

Antigen Details

Structure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function: Adhesion.
Ligand/Receptor: PSGL-1, CD43, CD206 and CD227.
Cell Type: Macrophages
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
Gene ID: 20612 View all products for this Gene ID
UniProt: View information about CD169 on UniProt.org

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