Brilliant Violet 605™ anti-mouse CD169 (Sigl-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3504	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Native Sialoadhesin from spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections¹, and spatial biology (IBEX)^4,5.

Application References (PubMed link indicates BioLegend citation)

Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
Chtanova T, et al. 2008. Immunity 29:487.
Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Kim EH, et al. 2020. Elife. 9:00. PubMed
Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
Wu X, et al. 2015. J Biol Chem. 290: 23135-23147. PubMed
Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed

RRID

AB_2564030 (BioLegend Cat. No. 142413)

Antigen Details

Structure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function: Adhesion.
Ligand/Receptor: PSGL-1, CD43, CD206 and CD227.
Cell Type: Macrophages
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
Gene ID: 20612 View all products for this Gene ID
UniProt: View information about CD169 on UniProt.org

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