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APC anti-mouse CD169 (Siglec-1) Antibody
产品名称：
APC anti-mouse CD169 (Siglec-1) Antibody
产品类别：
抗体
产品编号：
142417
产品应用：
142417
[价格]
规格 价格 库存
25µg ￥ 1932 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Native Sialoadhesin from spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
  2. Chtanova T, et al. 2008. Immunity 29:487.
  3. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
  4. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  5. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  2. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  3. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  4. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  5. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  6. Kalafati L, et al. 2020. Cell. 183:771. PubMed
RRID
AB_2565640 (BioLegend Cat. No. 142417) AB_2565641 (BioLegend Cat. No. 142418)

Antigen Details

Structure
Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution

Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.

Function
Adhesion.
Ligand/Receptor
PSGL-1, CD43, CD206 and CD227.
Cell Type
Macrophages
Biology Area
Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References

1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.

Gene ID
20612 View all products for this Gene ID
UniProt
View information about CD169 on UniProt.org

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