Alexa Fluor® 647 anti-mouse CD169 (Siglec-1) A-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2052	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Native Sialoadhesin from spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. ?For immunohistochemisty on frozen tissue sections, a concentration range of 1-5 μg/mL is suggested.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections¹, and spatial biology (IBEX)^4,5.

Application References (PubMed link indicates BioLegend citation)

Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
Chtanova T, et al. 2008. Immunity 29:487.
Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Singh R, et al. 2021. Bio Protoc. 11:e3878. PubMed
Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed
Uderhardt S, et al. 2019. Cell. 177:541. PubMed
Le Gars M, et al. 2016. J Immunol. 197: 3225 - 3232. PubMed
Freeman SA, et al. 2020. Science. 367:301. PubMed
Gbotosho OT, et al. 2020. Exp Hematol. 19:84. PubMed
Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
Baratin M, et al. 2017. Immunity. 47:349. PubMed
Rojo R, et al. 2019. Nat Commun. 10:3215. PubMed
Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed

RRID

AB_2563620 (BioLegend Cat. No. 142407) AB_2563621 (BioLegend Cat. No. 142408)

Antigen Details

Structure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function: Adhesion.
Ligand/Receptor: PSGL-1, CD43, CD206 and CD227.
Cell Type: Macrophages
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
Gene ID: 20612 View all products for this Gene ID
UniProt: View information about CD169 on UniProt.org

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