Purified anti-BrdU Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 780	1

Product Details

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Iodouridine-conjugated ovalbumin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ICFC - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.

Application References (PubMed link indicates BioLegend citation)

Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
Hirota K, et al. 2007. J. Exp. Med. 204:41.
Godebu E, et al. 2008. J. Immunol. 181:1798.
Waskow C, et al. 2008. Nat. Immunol. 9:676.

Product Citations

Shao F, et al. 2021. Nat Commun. 12:6540. PubMed
Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
Sakurai Y, et al. 2018. Mol Ther Oncolytics. 11:102. PubMed

RRID

AB_2564497 (BioLegend Cat. No. 364101) AB_2564498 (BioLegend Cat. No. 364102)

Antigen Details

Structure: Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution: Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.
Function: The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family: Nuclear Markers
Antigen References: 1. Barker JM, et al. 2013. PLoS One 8:e63692.
2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205.
3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855.
4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954.
5. van der Wath RC, et al. 2009. PLoS One 4:e6972.
6. Dolbeare F, et al. 1985. Cytometry 6:521.
7. Gratzner HG. 1982. Science 218:474.
Gene ID: NA
UniProt: View information about BrdU on UniProt.org

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