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首页 > 抗体蛋白 > 抗体
FITC anti-BrdU Antibody
产品名称：
FITC anti-BrdU Antibody
产品类别：
抗体
产品编号：
364103
产品应用：
364103
[价格]
规格 价格 库存
25tests ￥ 1068 1

产品详情

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Iodouridine-conjugated ovalbumin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.

Application References

(PubMed link indicates BioLegend citation)
  1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
  2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
  3. Godebu E, et al. 2008. J. Immunol. 181:1798.
  4. Waskow C, et al. 2008. Nat. Immunol. 9:676.
Product Citations
  1. Chen T, et al. 2016. J Exp Med. 13(13):3025-3039. PubMed
  2. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  3. Skalniak L, et al. 2019. FEBS J. 286:1360. PubMed
  4. Endres T, et al. 2021. Molecular Cell. 81(4):830-844.e13. PubMed
  5. Skalniak L, et al. 2018. Cancers (Basel). 10:. PubMed
  6. Pettinati I, et al. 2018. Elife. 7:. PubMed
  7. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  8. Dong S, et al. 2021. Nature. 591:117. PubMed
  9. Bainor AJ et al. 2018. Cell reports. 25(10):2797-2807 . PubMed
  10. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  11. ter Huurne M, et al. 2017. Cell Stem Cell. 1.186805556. PubMed
  12. Narain A, et al. 2021. Molecular Cell. 81(15):3110-3127.e14. PubMed
  13. Geeraerts SL, et al. 2020. Molecular Cancer Therapeutics. 20(1):50-63. PubMed
  14. Katarzyna Magiera et al. 2017. Cell chemical biology. 24(4):458-470 . PubMed
  15. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  16. Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed
  17. Xu J, et al. 2021. Cell Death Dis. 12:381. PubMed
  18. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  19. Wang X, et al. 2022. Elife. 11:. PubMed
  20. Song L, et al. 2021. J Invest Dermatol. 141:903. PubMed
  21. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  22. Yang L, et al. 2021. Breast Cancer Res. 23:89. PubMed
  23. El Touny LH, et al. 2021. Oncotarget. 12:2114. PubMed
  24. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  25. Qi G, et al. 2021. Am J Cancer Res. 11:389. PubMed
RRID
AB_2564480 (BioLegend Cat. No. 364103) AB_2564481 (BioLegend Cat. No. 364104)

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution

Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.

Function
The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Barker JM, et al. 2013. PLoS One 8:e63692.
2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205.
3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855.
4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954.
5. van der Wath RC, et al. 2009. PLoS One 4:e6972.
6. Dolbeare F, et al. 1985. Cytometry 6:521.
7. Gratzner HG. 1982. Science 218:474.

Gene ID
NA
UniProt
View information about BrdU on UniProt.org

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