Alexa Fluor® 647 anti-BrdU Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1344	1

Product Details

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Iodouridine-conjugated ovalbumin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.

Application References (PubMed link indicates BioLegend citation)

Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
Hirota K, et al. 2007. J. Exp. Med. 204:41.
Godebu E, et al. 2008. J. Immunol. 181:1798.
Waskow C, et al. 2008. Nat. Immunol. 9:676.

Product Citations

Kaymak A, et al. 2018. Oncotarget. 9:28666. PubMed
Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
Casar Tena T, et al. 2019. Nucleic Acids Res. 47:134. PubMed
Li X, et al. 2018. Immunity. 48:530. PubMed
Avila A, et al. 2016. Nucleic Acids Res. 44: 6252 - 6261. PubMed

RRID

AB_2566451 (BioLegend Cat. No. 364107) AB_2566452 (BioLegend Cat. No. 364108)

Antigen Details

Structure: Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution: Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.
Function: The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family: Nuclear Markers
Antigen References: 1. Barker JM, et al. 2013. PLoS One 8:e63692.
2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205.
3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855.
4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954.
5. van der Wath RC, et al. 2009. PLoS One 4:e6972.
6. Dolbeare F, et al. 1985. Cytometry 6:521.
7. Gratzner HG. 1982. Science 218:474.
Gene ID: NA
UniProt: View information about BrdU on UniProt.org

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