PE anti-mouse Ly49H Antibody
- 产品名称：
- PE anti-mouse Ly49H Antibody
- 产品类别：
- 抗体
- 产品编号：
- 144705
- 产品应用：
- 144705
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C1498-Ly49A/H transfectant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity.? The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 144717 & 144718).
- Application References
(PubMed link indicates BioLegend citation) -
- Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP)
- Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block)
- Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)
- Product Citations
-
- Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
- Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
- Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
- RRID
- AB_2561673 (BioLegend Cat. No. 144705) AB_2561674 (BioLegend Cat. No. 144706)
- Structure
- 28.3 kD
- Distribution
-
NK cells but not NKT cells
- Function
- Interacts with m157, a cytomegalovirus-encoded protein
- Ligand/Receptor
- m157
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.
- Gene ID
- 16639 View all products for this Gene ID
- UniProt
- View information about Ly49H on UniProt.org
