Alexa Fluor® 647 anti-mouse Ly49H Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C1498-Ly49A/H transfectant

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipation¹, and in vitro² and in vivo³ blocking activity.? The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 144717 & 144718).

Application References (PubMed link indicates BioLegend citation)

Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP)
Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block)
Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)

Product Citations

Ranganathan P, et al. 2013. J Am Soc Nephrol. 25:239. PubMed

RRID

AB_2561745 (BioLegend Cat. No. 144709) AB_2561746 (BioLegend Cat. No. 144710)

Antigen Details

Structure: 28.3 kD
Distribution: NK cells but not NKT cells
Function: Interacts with m157, a cytomegalovirus-encoded protein
Ligand/Receptor: m157
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.
Gene ID: 16639 View all products for this Gene ID
UniProt: View information about Ly49H on UniProt.org

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