Purified anti-mouse CD25 (Maxpar® Ready) Antib
- 产品名称：
- Purified anti-mouse CD25 (Maxpar® Ready) Antib
- 产品类别：
- 抗体
- 产品编号：
- 101913
- 产品应用：
- 101913
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-2-dependent BALB/c mouse helper T-cell clone HT-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF? - Verified
- Recommended Usage
This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF?Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101925 and 101926).
- Additional Product Notes
Maxpar? is a registered trademark of Standard BioTools Inc.
- Application References
(PubMed link indicates BioLegend citation) -
- Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
- Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
- Product Citations
-
- Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
- Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Waickman AT, et al. 2017. Cytokine. 99:266. PubMed
- Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
- Wang Y, et al. 2021. Cancer Cell. :. PubMed
- Düsedau HP, et al. 2019. Glia. 67:193. PubMed
- Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
- Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
- Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed
- Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed
- Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
- Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
- Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
- Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
- Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
- Jun JC, et al. 2017. PLoS One. 12:e0180688. PubMed
- RRID
- AB_2562798 (BioLegend Cat. No. 101913)
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
-
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor, signal transduction
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
