FITC anti-mouse CD25 Antibody
- 产品名称：
- FITC anti-mouse CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 101907
- 产品应用：
- 101907
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-2-dependent BALB/c mouse helper T-cell clone HT-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF?Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101925 and 101926).
- Application References
(PubMed link indicates BioLegend citation) -
- Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
- Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
- Product Citations
-
- Chen N, et al. 2021. Front Immunol. 12:756423. PubMed
- Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
- Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
- Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
- Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
- Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
- Yang K, et al. 2022. J Clin Invest. 132:. PubMed
- Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
- Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
- Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
- Carbonnelle D, et al. 2009. J Pharmacol Exp Ther. 331:771. PubMed
- Snell LM, et al. 2018. Immunity. 49:678. PubMed
- Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
- Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
- Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
- Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
- Huang J, et al. 2014. J Immunol. 192:1972. PubMed
- van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed
- Shi R, et al. 2020. J Interferon Cytokine Res. 40:188. PubMed
- Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
- RRID
- AB_961210 (BioLegend Cat. No. 101907) AB_961212 (BioLegend Cat. No. 101908)
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
-
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor, signal transduction
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
