APC/Fire™ 750 anti-mouse CD25 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1908	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

IL-2-dependent BALB/c mouse helper T-cell clone HT-2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors^1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF?Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101925 and 101926).

Application References (PubMed link indicates BioLegend citation)

Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)

Product Citations

Imani J, et al. 2021. JCI Insight. 6:. PubMed

RRID

AB_2783040 (BioLegend Cat. No. 101921) AB_2783041 (BioLegend Cat. No. 101922)

Antigen Details

Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor, signal transduction
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
Gene ID: 16184 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

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