Alexa Fluor® 647 anti-mouse CD102 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD102 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105611
- 产品应用：
- 105611
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS cells transfected with mouse ICAM-2 cDNA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105608).
- Application References
(PubMed link indicates BioLegend citation) -
- Xu H, et al. 1996. J. Immunol. 156:4909.
- Gerwin N, et al. 1999. Immunity 10:9.
- Product Citations
-
- Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
- Regev O, et al. 2022. Front Immunol. 13:849701. PubMed
- Minutti CM, et al. 2019. Immunity. 50:645. PubMed
- Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
- De Niz M, et al. 2021. Cell Rep. 36:109741. PubMed
- Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
- De Niz M, et al. 2022. STAR Protoc. 3:101450. PubMed
- Souza C, et al. 2012. J Biol Chem. 287:43810. PubMed
- RRID
- AB_2122183 (BioLegend Cat. No. 105611) AB_2122182 (BioLegend Cat. No. 105612)
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
- Function
- Lymphocyte recirculation, costimulation
- Ligand/Receptor
- LFA-1, Mac-1
- Cell Type
- Endothelial cells, Lymphocytes, Monocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017.
3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
- Gene ID
- 15896 View all products for this Gene ID
- UniProt
- View information about CD102 on UniProt.org
