Alexa Fluor® 488 anti-mouse CD102 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD102 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105609
- 产品应用：
- 105609
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS cells transfected with mouse ICAM-2 cDNA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105608).
- Application References
(PubMed link indicates BioLegend citation) -
- Xu H, et al. 1996. J. Immunol. 156:4909.
- Gerwin N, et al. 1999. Immunity 10:9.
- Product Citations
-
- Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
- Halai K, et al. 2014. J Cell Sci. 127:620. PubMed
- Watari K, et al. 2020. Commun Biol. 3:107. PubMed
- Sung SJ, et al. 2020. Bio Protoc. 10:e3663. PubMed
- He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
- Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed
- RRID
- AB_2264501 (BioLegend Cat. No. 105609)
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
- Function
- Lymphocyte recirculation, costimulation
- Ligand/Receptor
- LFA-1, Mac-1
- Cell Type
- Endothelial cells, Lymphocytes, Monocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017.
3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
- Gene ID
- 15896 View all products for this Gene ID
- UniProt
- View information about CD102 on UniProt.org
