TotalSeq™-C0581 anti-human TCR Vα7.2 A-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant TCR

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-C oligomer under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 ?segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells.?Additional reported applications (for the relevant formats) include: spatial biology (IBEX)^3,4.

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Martin E, et al. 2009. PLoS Biol. 7:525.
Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Shangguan S, et al. 2021. Elife. 10:. PubMed

RRID

AB_2810556 (BioLegend Cat. No. 351735)

Antigen Details

Structure: Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
Distribution: Mucosal-associated invariant T (MAIT) cells and conventional T cells
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
Gene ID: 6955 View all products for this Gene ID
UniProt: View information about TCR Valpha7.2 on UniProt.org

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