Purified anti-human TCR Vα7.2 Antibody
- 产品名称：
- Purified anti-human TCR Vα7.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 351702
- 产品应用：
- 351702
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant TCR
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 ?segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells.?Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
- Application References
(PubMed link indicates BioLegend citation) -
- Martin E, et al. 2009. PLoS Biol. 7:525.
- Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
- Shaler C, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-2050-7. PubMed
- Wakao H, et al. 2013. Cell Stem Cell. 12:1. PubMed
- Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
- Ye G, et al. 2021. Stem Cell Reports. 16:926. PubMed
- Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
- Marrero I, et al. 2020. Alcohol Clin Exp Res. 44:1061. PubMed
- Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
- Jiang H, et al. 2022. Clin Transl Med. 12:e730. PubMed
- Nicol B,et al. 2017. J Autoimmun. . 10.1016/j.jaut.2017.10.005. PubMed
- RRID
- AB_10900258 (BioLegend Cat. No. 351702)
- Structure
- Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
- Distribution
-
Mucosal-associated invariant T (MAIT) cells and conventional T cells
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
- Gene ID
- 6955 View all products for this Gene ID
- UniProt
- View information about TCR Valpha7.2 on UniProt.org
