PE/Cyanine7 anti-human TCR Vα7.2 Antibody
- 产品名称：
- PE/Cyanine7 anti-human TCR Vα7.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 351711
- 产品应用：
- 351711
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant TCR
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 ?segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells.?Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Martin E, et al. 2009. PLoS Biol. 7:525.
- Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Marquardt I, et al. 2022. Front Microbiol. 12:752549. PubMed
- Wakao H, et al. 2013. Cell Stem Cell. 12:1. PubMed
- Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
- Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
- Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
- Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
- Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed
- Leng T, et al. 2019. Cell Rep. 28:3077. PubMed
- Wilgenburg B, et al. 2016. Nat Commun. 7:11653. PubMed
- Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
- RRID
- AB_2561993 (BioLegend Cat. No. 351711) AB_2561994 (BioLegend Cat. No. 351712)
- Structure
- Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
- Distribution
-
Mucosal-associated invariant T (MAIT) cells and conventional T cells
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
- Gene ID
- 6955 View all products for this Gene ID
- UniProt
- View information about TCR Valpha7.2 on UniProt.org
