TotalSeq™-A1013 anti-mouse CD365 (Tim-1) Ant-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse TIM-1 (igV domain) FC chimera IFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq?-A oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.
Additional Product Notes: TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CCTTGGCACCCGAGAATTCCA (PCR handle), and BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A (capture sequence). B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.
RRID: AB_2832534 (BioLegend Cat. No. 151703)

Antigen Details

Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain with the predicted molecular weight of 33 kD.
Distribution: Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4⁺ lymphocytes, especially Th2 cell lines.
Function: Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Ligand/Receptor: Binds hepatitis A virus in humans, Tim-4, and LMIR5/CD300b.
Biology Area: Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. McIntire JJ, et al. 2001. Nat. Immunol. 2:1109.
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69.
4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455.
5. Umetsu S, et al. 2005. Nat. Immunol. 6:447.
6. Lee HH, et al. 2010. J. Immunol. 185:5225.
Gene ID: 171283 View all products for this Gene ID
UniProt: View information about CD365 on UniProt.org

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