Purified anti-Alix Antibody
- 产品名称：
- Purified anti-Alix Antibody
- 产品类别：
- 抗体
- 产品编号：
- 634501
- 产品应用：
- 634501
- Verified Reactivity
- Human, Mouse, and Frog
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full-length human Alix protein
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 ?g/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 3A9 has been shown to be useful for Western blotting1, immunoprecipitation, and immunofluorescence2 staining of the human and mouse Alix protein.
Clone 3A9 shows strong specificity for mouse Alix.
- Application References
(PubMed link indicates BioLegend citation) -
- Morel L, et al. 2013. J Biol Chem. 288:7105. (WB) PubMed
- Pan S, et al. 2008. EMBO J. 27:2077. (IF)
- Ertunc ME, et al. 2015. J Lipid Res. 56:423. PubMed
- Product Citations
-
- Yang C, et al. 2011. PLoS One. 6:e22517. PubMed
- Jia J, et al. 2020. Dev Cell. 69:52. PubMed
- Suthar J, et al. 2020. Anal Chem. 92:4082. PubMed
- Gulluni F, et al. 2021. Science. 374:eabk0410. PubMed
- Gorji-Bahri G, et al. 2021. Cell Biol Int. 45:1098. PubMed
- Morel L, et al. 2013. J Biol Chem. 288:7105. PubMed
- Yoon S, et al. 2017. Immunity. 47:51:00. PubMed
- Valkov N, et al. 2021. Life Sci Alliance. 4:. PubMed
- Baba T, et al. 2021. Cell Death Dis. 12:322. PubMed
- Hüttenhain R et al. 2019. Cell Host Microbe. 26(1):86-99 . PubMed
- Moore D, et al. 2019. Stem Cells Int. 2019:8710180. PubMed
- Davis OB, et al. 2020. Developmental Cell. 56(3):260-276.e7. PubMed
- G?rtner K, et al. 2018. Cancer Med. 11:39. PubMed
- Ko J, et al. 2018. Lab Chip. 3.261805556. PubMed
- Gorji-Bahri G, et al. 2021. J Cell Biochem. 122:425. PubMed
- Skowyra ML, et al. 2018. Science. 360:6384. PubMed
- Andre-Gregoire G, et al. 2022. iScience. 25:105118. PubMed
- Gunasekaran M, et al. 2020. J Heart Lung Transplant. 39:379. PubMed
- Hiraga C, et al. 2021. Sci Rep. 11:3326. PubMed
- Chand S, et al. 2021. J Extracell Vesicles. 10:e12177. PubMed
- Claude-Taupin A, et al. 2021. Nat Cell Biol. 23:846. PubMed
- Ertunc M, et al. 2015. J Lipid Res. 56:423. PubMed
- Millarte V, et al. 2022. EMBO Rep. 23:e53429. PubMed
- Jia J, et al. 2020. Mol Cell. 951:77. PubMed
- B?k R, et al. 2016. J Immunol Methods. 438:11-20. PubMed
- Gunasekaran M, et al. 2018. J Immunol. 200:2535. PubMed
- Schiller LT, et al. 2018. Mol Ther Methods Clin Dev. 15:1630. PubMed
- RRID
- AB_2268110 (BioLegend Cat. No. 634501) AB_2162471 (BioLegend Cat. No. 634502)
- Structure
- Belongs to a conserved family of proteins that have in common an N-terminal Bro1 domain and a C-terminal proline-rich domain. Predicted molecular weight approximately 96 kD.
- Distribution
-
Cytoplasm. Alix is also abundant within extracellular vesicles known as exosomes, and enriched in virions that are released from cells under the direction of the HIV-1 Gag protein.
- Function
- Adaptor in signal transduction pathways. Involved in apoptosis and endosomal membrane trafficking and viral budding.
- Interaction
- ALG2, SH3GL2, H-Vps28, CHMP4B, CHMP4A, CHMP4C, SETA, CIN85, Src
- Biology Area
- Cell Biology
- Antigen References
-
1. Wu Y, et al. 2000. Differentiation 67:139.
2. Missotten M, et al. 1999. Cell Death Differ. 6:124.
3. Odorizzi G. 2006. J Cell Sci. 119:3025.
- Gene ID
- 10015 View all products for this Gene ID
- UniProt
- View information about Alix on UniProt.org
