Alexa Fluor® 594 anti-Alix Antibody
- 产品名称：
- Alexa Fluor® 594 anti-Alix Antibody
- 产品类别：
- 抗体
- 产品编号：
- 634504
- 产品应用：
- 634504
- Verified Reactivity
- Human, Mouse, and Frog
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full-length human Alix protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
Clone 3A9 has been shown to be useful for Western blotting1, immunoprecipitation, and immunofluorescence2 staining of the human and mouse Alix protein.
Clone 3A9 shows strong specificity for mouse Alix.
- Application References
(PubMed link indicates BioLegend citation) -
- Morel L, et al. 2013. J Biol Chem. 288:7105. (WB) PubMed
- Pan S, et al. 2008. EMBO J. 27:2077. (IF)
- Ertunc ME, et al. 2015. J Lipid Res. 56:423. PubMed
- Product Citations
-
- Salybekov AA, et al. 2021. Front Cardiovasc Med. 8:754254. PubMed
- RRID
- AB_2565615 (BioLegend Cat. No. 634504)
- Structure
- Belongs to a conserved family of proteins that have in common an N-terminal Bro1 domain and a C-terminal proline-rich domain. Predicted molecular weight approximately 96 kD.
- Distribution
-
Cytoplasm. Alix is also abundant within extracellular vesicles known as exosomes, and enriched in virions that are released from cells under the direction of the HIV-1 Gag protein.
- Function
- Adaptor in signal transduction pathways. Involved in apoptosis and endosomal membrane trafficking and viral budding.
- Interaction
- ALG2, SH3GL2, H-Vps28, CHMP4B, CHMP4A, CHMP4C, SETA, CIN85, Src
- Biology Area
- Cell Biology
- Antigen References
-
1. Wu Y, et al. 2000. Differentiation 67:139.
2. Missotten M, et al. 1999. Cell Death Differ. 6:124.
3. Odorizzi G. 2006. J Cell Sci. 119:3025.
- Gene ID
- 10015 View all products for this Gene ID
- UniProt
- View information about Alix on UniProt.org
