Ultra-LEAF™ Purified anti-mouse CD31 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2508	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C3H/HeJ mouse hematopoietic progenitor cell line 3

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.⁸ Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, and immunohistochemical staining^5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References (PubMed link indicates BioLegend citation)

Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
Chacko AM, et al. 2012. PLoS One 7:e34958.
Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed

RRID

AB_2832290 (BioLegend Cat. No. 102445) AB_2832291 (BioLegend Cat. No. 102446)

Antigen Details

Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, α_V/β₃ integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.
Gene ID: 18613 View all products for this Gene ID
UniProt: View information about CD31 on UniProt.org

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