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首页 > 抗体蛋白 > 抗体
Purified anti-mouse CD31 Antibody
产品名称：
Purified anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102401
产品应用：
102401
[价格]
规格 价格 库存
50µg ￥ 648 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  2. Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
  3. Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed
  4. Hata E, et al. 2016. Int Immunol. 28: 283 - 292. PubMed
  5. Greineder C, et al. 2013. PLoS One. 14:80110. PubMed
  6. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  7. Dubrot J, et al. 2018. Life Sci Alliance. 1:e201800164. PubMed
  8. Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed
  9. Gong F, et al. 2020. Nat Commun. 3.036111111. PubMed
  10. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  11. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  12. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  13. Rodionov RN, et al. 2022. Sci Rep. 12:9381. PubMed
  14. Nessler I, et al. 2020. Cancer Res. 80:1268. PubMed
  15. Sun W, et al. 2021. Mol Ther. 29:1312. PubMed
  16. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  17. Molica F,et al. 2017. Sci Rep.. 10.1038/s41598-017-14130-4. PubMed
  18. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  19. Bertrand JA, et al. 2020. Invest Ophthalmol Vis Sci. 61:41:00. PubMed
  20. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  21. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  22. Yan SLS, et al. 2021. STAR Protoc. 2:100498. PubMed
  23. Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed
  24. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  25. González LA, et al. 2021. Front Immunol. 12:638917. PubMed
  26. Bhatnagar S, et al. 2018. Mol Pharm. 3. PubMed
  27. Alexandrushkina N, et al. 2020. Int J Mol Sci. 21:00. PubMed
  28. Khoa LTP, et al. 2020. STAR Protoc. 1:100136. PubMed
  29. Wang W, et al. 2018. J Vasc Surg. 68:1538. PubMed
  30. Subramanian BC, et al. 2020. J Cell Biol. 219:. PubMed
  31. Taniguchi Y, et al. 2020. PLoS One. 15:e0228203. PubMed
  32. Tamburini BAJ, et al. 2019. Front Immunol. 10:1036. PubMed
  33. Khera E, et al. 2021. Neoplasia. 1.104166667. PubMed
  34. Stepanova V, et al. 2016. J Biol Chem. 291: 15029 - 15045. PubMed
  35. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  36. Eslani M, et al. 2018. Stem Cells. 36:775. PubMed
  37. Fujimura N, et al. 2016. J Vasc Surg. 64: 46-54. PubMed
  38. Xiong S, et al. 2020. J Clin Invest. 130:3684. PubMed
  39. Murray CW, et al. 2022. Nat Commun. 13:1090. PubMed
  40. Lopes N, et al. 2017. EMBO Mol Med. 0.954861111. PubMed
  41. Cilliers C, et al. 2018. Cancer Res. 78:758. PubMed
RRID
AB_312896 (BioLegend Cat. No. 102401) AB_312897 (BioLegend Cat. No. 102402)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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