Purified anti-mouse CD31 (Maxpar® Ready) Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1272	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C3H/HeJ mouse hematopoietic progenitor cell line 3

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.⁸ Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, and immunohistochemical staining^5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
Chacko AM, et al. 2012. PLoS One 7:e34958.
Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed

RRID

AB_2563741 (BioLegend Cat. No. 102425)

Antigen Details

Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, α_V/β₃ integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.
Gene ID: 18613 View all products for this Gene ID
UniProt: View information about CD31 on UniProt.org

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