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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse CD31 Antibody
产品名称：
PerCP/Cyanine5.5 anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102419
产品应用：
102419
[价格]
规格 价格 库存
25µg ￥ 1488 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  2. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  3. Sun C, et al. 2020. Cell Stem Cell. 26(5):707-721. PubMed
  4. Lin J, et al. 2021. Front Immunol. 12:796260. PubMed
  5. Shen H, et al. 2022. Nat Commun. 13:6030. PubMed
  6. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  7. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  8. Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
  9. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  10. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  11. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  12. Li X, et al. 2022. Bone Res. 10:46. PubMed
  13. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  14. Parker KR, et al. 2020. Cell. 183(1):126-142.e17. PubMed
  15. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  16. Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed
  17. Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed
  18. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  19. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  20. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  21. Shan B, et al. 2020. Nat Metab. 1332:2. PubMed
  22. Francipane MG, et al. 2020. Am J Pathol. 190:252. PubMed
  23. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  24. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  25. Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed
  26. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  27. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  28. Tal R, et al. 2019. Biol Reprod. 100:1228. PubMed
  29. Wang Y, et al. 2020. Theranostics. 6.736111111. PubMed
  30. Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed
  31. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  32. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  33. Noll J, et al. 2014. Haematologica. 99:163. PubMed
RRID
AB_10612742 (BioLegend Cat. No. 102419) AB_10613644 (BioLegend Cat. No. 102420)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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