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Pacific Blue™ anti-mouse CD31 Antibody
产品名称：
Pacific Blue™ anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102421
产品应用：
102421
[价格]
规格 价格 库存
25µg ￥ 1488 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  2. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  3. Reinitz F, et al. 2022. Elife. 11:. PubMed
  4. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  5. Vesprey A, et al. 2021. J Bone Miner Res. 36:1000. PubMed
  6. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  7. Ogawa-Wong A, et al. 2022. Metabolites. 12:. PubMed
  8. Lee C, et al. 2014. Cancer Res. 74:341. PubMed
  9. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  10. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  11. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  12. Zhao X, et al. 2020. Methods Mol Biol. 2346:21. PubMed
  13. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  14. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  15. Farsaci B, et al. 2014. Cancer Immunol Res. 2:1090. PubMed
  16. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  17. Frech S, et al. 2021. J Invest Dermatol. . PubMed
  18. Jing Y, et al. 2020. Cancer Gene Ther. 27:910. PubMed
  19. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  20. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  21. An X, et al. 2020. Thyroid. . PubMed
  22. Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
  23. Xie X, et al. 2018. FASEB J. 32:1296. PubMed
  24. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  25. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  26. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  27. Peloquin GL, et al. 2019. Respir Res. 20:123. PubMed
  28. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  29. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  30. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_10613457 (BioLegend Cat. No. 102421) AB_10612926 (BioLegend Cat. No. 102422)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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