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首页 > 抗体蛋白 > 抗体
FITC anti-mouse CD31 Antibody
产品名称：
FITC anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102405
产品应用：
102405
[价格]
规格 价格 库存
50µg ￥ 1068 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Hattori Y, et al. 2022. J Neurosci. 42:362. PubMed
  2. Charoensaensuk V, et al. 2022. Antioxidants (Basel). 11:. PubMed
  3. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  4. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  5. Urban SK, et al. 2020. Liver Int. 40:3103. PubMed
  6. Sullivan D, Watson R, Muller W 2016. Am J Physiol Heart Circ Physiol. 311: H621 - H632. PubMed
  7. Yoshida Y, et al. 2015. J Mol Cell Cardiol. 85: 183-198. PubMed
  8. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  9. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  10. Stubelius A, et al. 2017. Immunobiology. 10.1016/j.imbio.2017.05.007. PubMed
  11. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  12. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  13. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  14. Charoensaensuk V, et al. 2021. Cells. 10:. PubMed
  15. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  16. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  17. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  18. Carrick FR, et al. 2021. Life (Basel). 11:. PubMed
  19. Diebold LP, et al. 2019. Nat Metab. 1:158. PubMed
  20. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  21. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  22. Wen A, et al. 2012. J Vis Exp. 69: 4352. PubMed
  23. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  24. Yong T, et al. 2019. Nat Commun. 10:3838. PubMed
  25. Kotla S, et al. 2019. JCI Insight. 4:9. PubMed
  26. Long JT, et al. 2022. Elife. 11:. PubMed
  27. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  28. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  29. Li CM, et al. 2020. Cell Reports. 33(13):108566. PubMed
  30. Yip HYK, et al. 2018. PLoS One. 3. PubMed
  31. Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed
  32. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  33. Sakai H, et al. 2022. iScience. 25:103928. PubMed
  34. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  35. Zhao Q, et al. 2020. Cancer Res. 80:5531. PubMed
  36. Hu J, et al. 2022. Stem Cell Res Ther. 13:349. PubMed
  37. Kasuga A, et al. 2020. Cancer Sci. . PubMed
  38. Usui-Ouchi A, et al. 2020. Proc Natl Acad Sci U S A. 28297:117. PubMed
RRID
AB_312900 (BioLegend Cat. No. 102405) AB_312901 (BioLegend Cat. No. 102406)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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