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Brilliant Violet 605™ anti-mouse CD31 Antibo
产品名称:
Brilliant Violet 605™ anti-mouse CD31 Antibo
产品类别:
抗体
产品编号:
102427
产品应用:
102427
[价格]
规格 价格 库存
50µg ¥ 3792 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  2. Nicolás-?vila J?, et al. 2021. STAR Protoc. 2:100286. PubMed
  3. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  4. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  5. Ibrahim A, et al. 2020. Cell Metabolism. 32(2):309-319.e7. PubMed
  6. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  7. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  8. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  9. De Simone G, et al. 2021. Immunity. :. PubMed
  10. Vink CS, et al. 2020. Cell Rep. 31:107627. PubMed
  11. Blriot C, et al. 2021. Immunity. :. PubMed
  12. Clark JT, et al. 2021. eLife. 10:00. PubMed
  13. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  14. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  15. Matthews O, et al. 2020. EBioMedicine. 62:103092. PubMed
  16. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  17. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  18. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  19. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
RRID
AB_2563982 (BioLegend Cat. No. 102427)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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