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Brilliant Violet 421™ anti-mouse CD31 Antibo
产品名称：
Brilliant Violet 421™ anti-mouse CD31 Antibo
产品类别：
抗体
产品编号：
102423
产品应用：
102423
[价格]
规格 价格 库存
125µL ￥ 2184 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g size: 0.2 mg/mL
?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC -?Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Dong W, et al. 2022. Theranostics. 12:4110. PubMed
  2. Dokun A, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 790-803. PubMed
  3. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  4. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  5. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  6. Quaife-Ryan GA, et al. 2020. Development. 147: . PubMed
  7. Kugler J, et al. 2015. Toxicol Sci. 146: 374-385. PubMed
  8. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
  9. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  10. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  11. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  12. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  13. Redpath AN, et al. 2021. STAR Protocols. 2(1):100359. PubMed
  14. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  15. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  16. Ayari A, et al. 2020. Commun Biol. 3:237. PubMed
  17. Ma Z, et al. 2022. Methods Mol Biol. 2471:49. PubMed
  18. Wakabayashi T, et al. 2022. Invest Ophthalmol Vis Sci. 63:5. PubMed
  19. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  20. Chuang CH, et al. 2020. Cancer Research. 81(3):567-579. PubMed
  21. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  22. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  23. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  24. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  25. Dong W, et al. 2020. Sci Rep. 10:6652. PubMed
  26. Ombrato L, et al. 2021. Nat Protoc. 16:872. PubMed
  27. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  28. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  29. Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
  30. Naito H, et al. 2019. Dev Cell. 48:151. PubMed
  31. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  32. Quaife-Ryan GA, et al. 2020. Development. . PubMed
RRID
AB_2562186 (BioLegend Cat. No. 102423) AB_2650892 (BioLegend Cat. No. 102424)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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