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Biotin anti-mouse CD31 Antibody
产品名称：
Biotin anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102404
产品应用：
102404
[价格]
规格 价格 库存
500µg ￥ 3240 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  2. Tarban N, et al. 2022. Cells. 11:. PubMed
  3. Xie T, et al. 2021. Sci Adv. 7: . PubMed
  4. Larsen SB, et al. 2021. Cell Stem Cell. :. PubMed
  5. Verena Sigl et al. 2017. Cell research. 26(7):761-74 . PubMed
  6. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  7. Aneas I, et al. 2021. Nat Commun. 12:6115. PubMed
  8. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  9. Wagh A, et al. 2013. Nanomedicine. 9:449. PubMed
  10. Elsarraj H, et al. 2013. J Cell Sci. 126:2446. PubMed
  11. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  12. Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed
  13. Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed
  14. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  15. Lü?nd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  16. Gu VW, et al. 2021. Stem Cell Reports. 16:106. PubMed
  17. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  18. Budai Z, et al. 2021. Cells. 10:. PubMed
  19. Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed
  20. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  21. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  22. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  23. Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed
  24. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  25. De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed
  26. Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
  27. Kopinke D et al. 2017. Cell. 170(2):340-351 . PubMed
  28. Liu X, et al. 2021. iScience. 24(6):102551. PubMed
  29. Seong J, et al. 2018. Development. 145:14. PubMed
RRID
AB_312899 (BioLegend Cat. No. 102404)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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