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APC anti-mouse CD31 Antibody
产品名称：
APC anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102409
产品应用：
102409
[价格]
规格 价格 库存
25µg ￥ 1356 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Yang D, et al. 2022. Cell. 185:1905. PubMed
  2. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  3. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  4. Wang J, et al. 2019. Front Immunol. 9:3157. PubMed
  5. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  6. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
  7. Park M, et al. 2021. BMB Rep. 54:470. PubMed
  8. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
  9. Srivastava N, et al. 2020. FASEB J. 34:12805. PubMed
  10. Hesse J, et al. 2021. eLife. 10:00. PubMed
  11. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  12. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  13. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  14. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
  15. Quintana E, et al. 2012. Sci Transl Med. 149:159. PubMed
  16. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  17. Eskiocak U, et al. 2016. Nat Commun. 7:12336. PubMed
  18. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  19. Zeng Z, et al. 2022. Oncogene. :. PubMed
  20. Chu X, et al. 2021. Front Cell Dev Biol. 9:671841. PubMed
  21. Long JT, et al. 2022. Elife. 11:. PubMed
  22. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  23. Sarvi S et al. 2018. Cell chemical biology. 25(12):1456-1469 . PubMed
  24. Yang T, et al. 2022. Antioxidants (Basel). 11:. PubMed
  25. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  26. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  27. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  28. Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
  29. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  30. Yeo SK, et al. 2020. eLife. 9:e58810.. PubMed
  31. Hendee K, et al. 2021. Aging (Albany NY). 13:7781. PubMed
  32. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
  33. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  34. Kalinina A, et al. 2022. PLoS One. 17:e0250752. PubMed
  35. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  36. Kim KS, et al. 2022. JCI Insight. 7:. PubMed
  37. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  38. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  39. Shen B, et al. 2019. Elife. 8:e42274. PubMed
  40. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  41. Pal B, et al. 2021. Breast Cancer Res. 23:69. PubMed
  42. Ouadah Y, et al. 2019. Cell. 179:403. PubMed
  43. Dumm RE, et al. 2020. Cell Reports. 32(9):108103.. PubMed
  44. Longchamp A, et al. 2018. Cell. 173:117. PubMed
RRID
AB_312904 (BioLegend Cat. No. 102409) AB_312905 (BioLegend Cat. No. 102410)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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