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Alexa Fluor® 647 anti-mouse CD31 Antibody
产品名称：
Alexa Fluor® 647 anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102415
产品应用：
102415
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Wan P, et al. 2021. Neurophotonics. 8:035007. PubMed
  2. Miyawaki T, et al. 2020. Nat Commun. 1.225. PubMed
  3. Soveg F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L573-L583. PubMed
  4. McQualter J, et al. 2009. Stem Cells. 27:623. PubMed
  5. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  6. Berdnikovs S, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 304:240. PubMed
  7. Zhu J, et al. 2020. Adv Sci (Weinh). 7:1903185. PubMed
  8. Plant T, et al. 2020. J Clin Invest. 130:3221. PubMed
  9. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  10. Qi Y, et al. 2019. Sci Adv. 5:eaau8355. PubMed
  11. Graney PL, et al. 2020. Sci Adv. 6:eaay6391. PubMed
  12. Matryba P, et al. 2020. J Biophotonics. 13:e202000072. PubMed
  13. Akram KM, et al. 2019. Nat Commun. 10:1178. PubMed
  14. De Niz M, et al. 2021. Cell Rep. 36:109741. PubMed
  15. Jagannathan P, et al. 2017. Sci Rep. 10.1038/s41598-017-10624-3. PubMed
  16. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  17. Zotter B, et al. 2022. J Neurosci. 42:183. PubMed
  18. Roncato F, et al. 2021. Cell Adhesion Migration. 15(1):166-179. PubMed
  19. Michael BD, et al. 2020. Cell Reports. 32(11):108150. PubMed
  20. Moretti FA, et al. 2018. Elife. 7:e35816. PubMed
  21. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  22. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  23. Lü?nd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  24. Prizant H, et al. 2021. Cell Reports. 36(6):109523. PubMed
  25. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
  26. Esposito M, et al. 2019. Nat Cell Biol. 21:627. PubMed
  27. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  28. Mori Y, et al. 2014. Sci Rep. 4:6997. PubMed
  29. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  30. Park JK, et al. 2018. Stem Cell Res Ther. 9:197. PubMed
  31. Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed
  32. Stalin J, et al. 2016. Oncogene. 10.1038/onc.2016.83. PubMed
  33. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  34. Lui BG, et al. 2020. Nat Commun. 0.663194444. PubMed
  35. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  36. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  37. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  38. De Niz M, et al. 2022. STAR Protoc. 3:101450. PubMed
  39. Riedel S, et al. 2012. PLoS One. 7:e52398. PubMed
  40. Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed
  41. Oni TE, et al. 2020. J Exp Med. :217. PubMed
  42. Dougan M, et al. 2018. Cancer Immunol Res. 6:389. PubMed
RRID
AB_493411 (BioLegend Cat. No. 102415) AB_493410 (BioLegend Cat. No. 102416)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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