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Alexa Fluor® 488 anti-mouse CD31 Antibody
产品名称:
Alexa Fluor® 488 anti-mouse CD31 Antibody
产品类别:
抗体
产品编号:
102413
产品应用:
102413
[价格]
规格 价格 库存
25µg ¥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Kazakevych J, et al. 2020. Genome Biol. 21:64. PubMed
  2. Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed
  3. Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
  4. Watanabe S, et al. 2015. J Virol. 89:5847. PubMed
  5. Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed
  6. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  7. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  8. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  9. Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed
  10. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
  11. Melhem H, et al. 2022. Mucosal Immunol. 15:443. PubMed
  12. Kirkby N, et al. 2012. Proc Natl Acad Sci U S A. 109:17597. PubMed
  13. Fan Z, et al. 2021. Cell Metabolism. :. PubMed
  14. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  15. Kazakevych J, et al. 2019. Sci Rep. 9:10410. PubMed
  16. Losenkova K, et al. 2020. J Cell Sci. 133:00:00. PubMed
  17. Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed
  18. Lupan AM, et al. 2021. Aging (Albany NY). 13:15875. PubMed
  19. Namachivayam K, et al. 2020. Proc Natl Acad Sci U S A. 117:10958. PubMed
  20. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
RRID
AB_493409 (BioLegend Cat. No. 102413) AB_493408 (BioLegend Cat. No. 102414)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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