FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections.
Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).
B cell and activated T cells, APCs of H-2d?mice
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Wall KA, et al. 1983. J. Immunol. 131:1056.
4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267.