KIRAVIA Blue 520™ anti-mouse CD28 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1980	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Syrian Hamster

Immunogen

C57BL/6 mouse T-cell lymphoma EL-4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520? under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* KIRAVIA Blue 520? has an excitation maximum of 495 nm, and a maximum emission of 520 nm.

KIRAVIA Blue? 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Sony and the Sony logo are registered trademarks of Sony Corporation.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro costimulation of T and NK cells¹, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity^3,4, in vitro induction of thymocyte differentiation^2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) (Cat. No. 102116).

Application References (PubMed link indicates BioLegend citation)

Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
Cibotti R, et al. 1997. Immunity 6:245. (Costim)
Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
Perchonock CE, et al. 2007. J. Immunol. 179:1768.
Barbi J, et al. 2007. Blood 110:2215.
Milpied P, et al. 2011. Blood 118:2993. PubMed
Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
Li CR, et al. 2014. J Immunol. 192:1425. PubMed
Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed

RRID

AB_2890669 (BioLegend Cat. No. 102135) AB_2890669 (BioLegend Cat. No. 102136)

Antigen Details

Structure: Ig superfamily, 44 kD
Distribution: Thymocytes, CD4⁺, CD8⁺ peripheral T cells, NK cells
Function: Costimulates T and NK cells
Ligand/Receptor: CD80 (B7-1), CD86 (B7-2)
Cell Type: NK cells, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.
Gene ID: 12487 View all products for this Gene ID
UniProt: View information about CD28 on UniProt.org

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