PE anti-human IL-1α Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 2484	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human IL-1α

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

?g sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

ELISA or ELISPOT Capture¹: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF? purified antibody (Cat. No. 500108) is suggested for ELISPOT capture.
Flow Cytometry⁴: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunohistochemistry³. The 364-3B3-14 is a non-neutralizing antibody.

Application References (PubMed link indicates BioLegend citation)

Thorpe R, et al. 1988. Lymphokine Res. 2:119.
Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177.
Lu Y, et al. 2015. PNAS. 112:607. PubMed

Product Citations

Aouba A, et al. 2010. Blood. 116:4070. PubMed

RRID

AB_315086 (BioLegend Cat. No. 500107) AB_315085 (BioLegend Cat. No. 500106)

Antigen Details

Structure: Cytokine; 17.5 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Cell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cell Targets: B cells, T cells, monocytes, dendritic cells
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York.
3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello, C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin
Gene ID: 3552 View all products for this Gene ID
UniProt: View information about IL-1alpha on UniProt.org

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