PE anti-human LT-α (TNF-β) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1548	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

E. coli expressed, recombinant human LT-α.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

ELISA or ELISPOT Detection^1,2: The biotinylated 359-81-11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 359-238-8 antibody (Cat. No. 503002/503004) as the capture antibody.
Flow Cytometry³: The fluorochrome-labeled 359-81-11 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify LT-α -producing cells within mixed cell populations. View intracellular cytokine staining protocol.
Neutralization^1,2: The 359-81-11 antibody can neutralize the bioactivity of natural or recombinant LT-α. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503108).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.

Application References (PubMed link indicates BioLegend citation)

Meager A, et al. 1987. J. Immunol. Methods 104:31.
Meager A, et al. 1987. Hybridoma. 6:305.
Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.

RRID

AB_315275 (BioLegend Cat. No. 503105)

Antigen Details

Structure: TNF superfamily; trimer; 25 kD (Mammalian).
Function: Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reactive oxygen, fibroblast proliferation.
Cell Sources: Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells.
Cell Targets: Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells.
Receptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Aggarwal, B., et al.Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc.
3. Bonavida, B., et al.Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel.
4. Paul, N., et al. 1987. Annu. Rev. Immunol. 6:407.
Regulation: Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric.
Gene ID: 4049 View all products for this Gene ID
UniProt: View information about LT-alpha on UniProt.org

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