Biotin anti-human LT-α (TNF-β) Antibody-上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3936	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

E. coli expressed, recombinant human LT-α.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested
ELISPOT Detection, ICFC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 ?g/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection^1,2: The biotinylated 359-81-11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 359-238-8 antibody (Cat. No. 503002/503004) as the capture antibody.
Flow Cytometry³: The fluorochrome-labeled 359-81-11 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify LT-α -producing cells within mixed cell populations. View intracellular cytokine staining protocol.
Neutralization^1,2: The 359-81-11 antibody can neutralize the bioactivity of natural or recombinant LT-α. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503108).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.

Application References (PubMed link indicates BioLegend citation)

Meager A, et al. 1987. J. Immunol. Methods 104:31.
Meager A, et al. 1987. Hybridoma. 6:305.
Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.

RRID

AB_315274 (BioLegend Cat. No. 503104)

Antigen Details

Structure: TNF superfamily; trimer; 25 kD (Mammalian).
Function: Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reactive oxygen, fibroblast proliferation.
Cell Sources: Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells.
Cell Targets: Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells.
Receptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Aggarwal, B., et al.Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc.
3. Bonavida, B., et al.Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel.
4. Paul, N., et al. 1987. Annu. Rev. Immunol. 6:407.
Regulation: Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric.
Gene ID: 4049 View all products for this Gene ID
UniProt: View information about LT-alpha on UniProt.org

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