FITC anti-mouse H-2D-上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3564	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

(C57BL/6 x DBA/2)F₁ mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity¹, immunoprecipitation^2,3, blocking of cell adhesion^4,5, and immunohistochemical staining of acetone-fixed frozen sections⁶.

This clone does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).

Application References (PubMed link indicates BioLegend citation)

Ozato K, et al. 1982. Transplantation 34:113.
McCluskey J, et al. 1985. Cell 40:247.
McCluskey J, et al. 1986. J. Immunol. 136:1472.
Kane KP. 1994. J. Exp. Med. 179:1011.
Daniels BF, et al. 1994. J. Exp. Med. 180:687.
Nikolic B, et al. 1999. J. Immunol. 162:3402.
Ma XT, et al. 2006. Cancer Research 66:1169.
Matsumoto N, et al. 1998. Immunity 8:245.

Product Citations

Ma X, et al. 2006. Cancer Res. 66:1169. PubMed
Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed

RRID

AB_313487 (BioLegend Cat. No. 110606)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2D^d haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14964 View all products for this Gene ID
UniProt: View information about H-2Dd on UniProt.org

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