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Alexa Fluor® 647 anti-mouse H-2K
产品名称：
Alexa Fluor® 647 anti-mouse H-2K
产品类别：
抗体
产品编号：
114712
产品应用：
114712
[价格]
规格 价格 库存
100µg ￥ 2748 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BDF Splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1982. Transplantation 34:113.
  2. Sester M, et al. 2000. J. Biol. Chem. 34:113.
Product Citations
  1. Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed
  2. Wu J, et al. 2009. Clin Cancer Res. 1.063888889. PubMed
  3. Dixit S, et al. 2018. Biomaterials. 159:130. PubMed
  4. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
RRID
AB_493063 (BioLegend Cat. No. 114712)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd/H-2Dd haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
  2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
  3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Kd/H-2Dd on UniProt.org

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