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Biotin anti-mouse DC Marker (33D1) Antibody
产品名称:
Biotin anti-mouse DC Marker (33D1) Antibody
产品类别:
抗体
产品编号:
124903
产品应用:
124903
[价格]
规格 价格 库存
50µg ¥ 852 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3 and immunohistochemical staining of acetone-fixed frozen sections4.
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Binding of clone 33D1 is calcium-dependent. We recommend staining in EDTA-free FACS buffer containing a source of Ca2+ ions.

Application References

(PubMed link indicates BioLegend citation)
  1. Crowley MT, et al. 1990. J. Immunol. Methods 133:55.
  2. Nussenzwig MC, et al. 1982. P. Natl. Acad. Sci. USA 79:161.
  3. Steinman RM, et al. 1983. J. Exp. Med. 157:613. (IF)
  4. Fischer HG, et al. 2000. J. Immunol. 164:4826. (IHC)
Product Citations
  1. Ding Z, Dahlin J 2016. Sci Rep. 6:28290. PubMed
  2. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  3. Liu D, et al. 2020. J Exp Med. 217:. PubMed
RRID
AB_1186157 (BioLegend Cat. No. 124903) AB_1186159 (BioLegend Cat. No. 124904)

Antigen Details

Structure
The antigen recognized by 33D1 is yet unknown.
Distribution

33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive.

Function
GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen.
Cell Type
Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about 33D1 on UniProt.org

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