Ultra-LEAF™ Purified anti-human Lymphotoxin -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human LT-βR Fc protein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
WB, IP, Apop - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, ELISA detection¹, induction of apoptosis². The biotinylated 31G4D8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified 36G9G3 antibody (Cat. No. 322101/322102) as capture antibody. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 322003/322004).

Application References (PubMed link indicates BioLegend citation)

Developer's communication
Chen M-C, et al. 2003. J. Biol. Chem. 278:16073.

RRID

AB_2832630 (BioLegend Cat. No. 322011) AB_2832631 (BioLegend Cat. No. 322012)

Antigen Details

Structure

Member of the TNF receptor superfamily; transmembrane receptor containing 3 TNF receptor domains, predicted molecular weight approximately 47 kD

Distribution

Highly expressed in lung, liver, and kidney. Moderate expression in heart and testes; weak expression in brain, thymus, spleen, and lymph nodes.

Function

Promotes apoptosis via TRAF3 and TRAF5, plays a role in the development of lymphoid organs

Interaction

Receptor for heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT. Also interacts with TRAF3, TRAF4, and TRAF5 as well as hepatitis C virus core protein

Cell Type

Embryonic Stem Cells

Biology Area

Immunology, Stem Cells

Molecular Family

Cytokine/Chemokine Receptors

Antigen References